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rabbit anti-mouse ca1 antibody  (Servicebio Inc)

 
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    Servicebio Inc rabbit anti-mouse ca1 antibody
    Establishment of <t>CA1</t> KI ApoE [−/−] C57BL/6 mice via the CRISPR/Cas9 gene re-editing technique. A. Diagram of the establishment of KI mice with CA1 cDNA inserts. An expression cassette with a CAG promoter and CA1-encoding gene was inserted into intron 1 of the Rosa26 locus in the C57BL/6 mouse chromosome via the CRISPR/Cas9 system. B. Representative PCR images showing the successful establishment of CA1-overexpressing KI mice. a. PCR with primer set 1 yielded one band of 296 bp in KI mice. b. PCR with primer set 2 yielded one band of 335 bp in KI mice. c. PCR with primer set 3 yielded one band of 245 bp in the KI mice. KI: knock-in mice; WT: wild type; M: molecular size marker of DNA.
    Rabbit Anti Mouse Ca1 Antibody, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti-mouse ca1 antibody/product/Servicebio Inc
    Average 90 stars, based on 1 article reviews
    rabbit anti-mouse ca1 antibody - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "ApoE [−/−] CA1-overexpressing knock-in mice aggravated atherosclerosis by increasing M1 macrophages"

    Article Title: ApoE [−/−] CA1-overexpressing knock-in mice aggravated atherosclerosis by increasing M1 macrophages

    Journal: Atherosclerosis Plus

    doi: 10.1016/j.athplu.2025.03.003

    Establishment of CA1 KI ApoE [−/−] C57BL/6 mice via the CRISPR/Cas9 gene re-editing technique. A. Diagram of the establishment of KI mice with CA1 cDNA inserts. An expression cassette with a CAG promoter and CA1-encoding gene was inserted into intron 1 of the Rosa26 locus in the C57BL/6 mouse chromosome via the CRISPR/Cas9 system. B. Representative PCR images showing the successful establishment of CA1-overexpressing KI mice. a. PCR with primer set 1 yielded one band of 296 bp in KI mice. b. PCR with primer set 2 yielded one band of 335 bp in KI mice. c. PCR with primer set 3 yielded one band of 245 bp in the KI mice. KI: knock-in mice; WT: wild type; M: molecular size marker of DNA.
    Figure Legend Snippet: Establishment of CA1 KI ApoE [−/−] C57BL/6 mice via the CRISPR/Cas9 gene re-editing technique. A. Diagram of the establishment of KI mice with CA1 cDNA inserts. An expression cassette with a CAG promoter and CA1-encoding gene was inserted into intron 1 of the Rosa26 locus in the C57BL/6 mouse chromosome via the CRISPR/Cas9 system. B. Representative PCR images showing the successful establishment of CA1-overexpressing KI mice. a. PCR with primer set 1 yielded one band of 296 bp in KI mice. b. PCR with primer set 2 yielded one band of 335 bp in KI mice. c. PCR with primer set 3 yielded one band of 245 bp in the KI mice. KI: knock-in mice; WT: wild type; M: molecular size marker of DNA.

    Techniques Used: CRISPR, Expressing, Knock-In, Marker

    Changes in the body weights of the AS model mice. CA1-overexpressing ApoE [−/−] mice and ordinary ApoE [−/−] mice were induced to AS with high-fat food. These patients were divided into the following groups: normal, AS model, AS model with MTZ treatment and AS model with MTZ preventive-treatment. Each group included 15 mice. Mice with CA1 overexpression generally had greater body weights than did those without CA1 overexpression. The weight of the mice with AS was generally greater than that of the mice without AS, and the weight of the mice with AS was reduced following MTZ treatment and MTZ-preventive treatment.
    Figure Legend Snippet: Changes in the body weights of the AS model mice. CA1-overexpressing ApoE [−/−] mice and ordinary ApoE [−/−] mice were induced to AS with high-fat food. These patients were divided into the following groups: normal, AS model, AS model with MTZ treatment and AS model with MTZ preventive-treatment. Each group included 15 mice. Mice with CA1 overexpression generally had greater body weights than did those without CA1 overexpression. The weight of the mice with AS was generally greater than that of the mice without AS, and the weight of the mice with AS was reduced following MTZ treatment and MTZ-preventive treatment.

    Techniques Used: Over Expression

    Sudan IV staining of mouse cardiac aorta tissues. The number and extent of accumulated lipids in the aorta were semiquantitatively analyzed by calculating lipid accumulation in the whole aorta. The lipid accumulation in the CA1-overexpressing mice was much greater than that in the mice without CA1 overexpression. MTZ treatment significantly decreased the quantity and volume of lipid accumulation. ∗∗ indicates P < 0.01, ∗∗∗ indicates P < 0.001, and ∗∗∗∗ indicates P < 0.0001.
    Figure Legend Snippet: Sudan IV staining of mouse cardiac aorta tissues. The number and extent of accumulated lipids in the aorta were semiquantitatively analyzed by calculating lipid accumulation in the whole aorta. The lipid accumulation in the CA1-overexpressing mice was much greater than that in the mice without CA1 overexpression. MTZ treatment significantly decreased the quantity and volume of lipid accumulation. ∗∗ indicates P < 0.01, ∗∗∗ indicates P < 0.001, and ∗∗∗∗ indicates P < 0.0001.

    Techniques Used: Staining, Over Expression

    HE staining of mouse cardiac aorta tissues. The extent and composition of the aortic lesions were semiquantified by calculating the surface plaque area across the entire aortic area. The number of aortic plaques and wall thickness in CA1-overexpressing mice were greater than those in mice without CA1 overexpression. ∗ indicates P < 0.05, ∗∗ indicates P < 0.01, ∗∗∗ indicates P < 0.001 and ∗∗∗∗ indicates P < 0.0001.
    Figure Legend Snippet: HE staining of mouse cardiac aorta tissues. The extent and composition of the aortic lesions were semiquantified by calculating the surface plaque area across the entire aortic area. The number of aortic plaques and wall thickness in CA1-overexpressing mice were greater than those in mice without CA1 overexpression. ∗ indicates P < 0.05, ∗∗ indicates P < 0.01, ∗∗∗ indicates P < 0.001 and ∗∗∗∗ indicates P < 0.0001.

    Techniques Used: Staining, Over Expression

    Oil Red O staining of mouse cardiac aorta tissues. The number and size of fat deposits were semiquantitatively analyzed. Fat deposition in cardiac aorta tissues was greater in CA1-overexpressing mice with AS than in mice with AS without CA1 overexpression. MTZ treatment significantly decreased the volume and number of fat deposits. ∗ indicates P < 0.05, ∗∗ indicates P < 0.01, ∗∗∗ indicates P < 0.001 and ∗∗∗∗ indicates P < 0.0001.
    Figure Legend Snippet: Oil Red O staining of mouse cardiac aorta tissues. The number and size of fat deposits were semiquantitatively analyzed. Fat deposition in cardiac aorta tissues was greater in CA1-overexpressing mice with AS than in mice with AS without CA1 overexpression. MTZ treatment significantly decreased the volume and number of fat deposits. ∗ indicates P < 0.05, ∗∗ indicates P < 0.01, ∗∗∗ indicates P < 0.001 and ∗∗∗∗ indicates P < 0.0001.

    Techniques Used: Staining, Over Expression

    Biochemical examination of mouse peripheral blood. Compared with those in healthy controls, HDL levels were significantly lower, and AI, LDL, TC and TG levels were elevated in AS mice. The levels of these indices were restored after MTZ treatment. Moreover, the HDL level was significantly lower and the levels of AI, LDL, TC and TG were greater in CA1-overexpressing mice than in ApoE [−/−] mice with normal CA1 expression, regardless of whether these mice were induced to develop AS or treated with MTZ. ∗ indicates P < 0.05, ∗∗ indicates P < 0.01, ∗∗∗ indicates P < 0.001 and ∗∗∗∗ indicates P < 0.0001.
    Figure Legend Snippet: Biochemical examination of mouse peripheral blood. Compared with those in healthy controls, HDL levels were significantly lower, and AI, LDL, TC and TG levels were elevated in AS mice. The levels of these indices were restored after MTZ treatment. Moreover, the HDL level was significantly lower and the levels of AI, LDL, TC and TG were greater in CA1-overexpressing mice than in ApoE [−/−] mice with normal CA1 expression, regardless of whether these mice were induced to develop AS or treated with MTZ. ∗ indicates P < 0.05, ∗∗ indicates P < 0.01, ∗∗∗ indicates P < 0.001 and ∗∗∗∗ indicates P < 0.0001.

    Techniques Used: Expressing

    Immunostaining of CA1 expression in mouse aortic tissues. Immunohistochemistry revealed CA1 expression (brown) in the aortic plaques of the animals with AS (↓). CA1 was also weakly expressed in aortic VSMCs (↓↓). Immunofluorescent immunohistochemistry revealed that CA1 levels were increased in the cardiac aorta tissues of CA1-overexpressing ApoE [−/−] mice, regardless of whether the KI mice had induced AS, compared with those of ordinary ApoE [−/−] mice. The quantified signal is normalized to the total cellularized area. ∗∗∗ indicates P < 0.001 and ∗∗∗∗ indicates P < 0.0001.
    Figure Legend Snippet: Immunostaining of CA1 expression in mouse aortic tissues. Immunohistochemistry revealed CA1 expression (brown) in the aortic plaques of the animals with AS (↓). CA1 was also weakly expressed in aortic VSMCs (↓↓). Immunofluorescent immunohistochemistry revealed that CA1 levels were increased in the cardiac aorta tissues of CA1-overexpressing ApoE [−/−] mice, regardless of whether the KI mice had induced AS, compared with those of ordinary ApoE [−/−] mice. The quantified signal is normalized to the total cellularized area. ∗∗∗ indicates P < 0.001 and ∗∗∗∗ indicates P < 0.0001.

    Techniques Used: Immunostaining, Expressing, Immunohistochemistry

    Calcification of mouse cardiac aorta tissues via Von Kossa staining. Extensive calcium deposition was detected in cardiac aorta tissues from CA1-overexpressing AS and AS mice following MTZ treatment. Little calcification was observed in the aortic tissues of ordinary ApoE [−/−] mice with induced AS.
    Figure Legend Snippet: Calcification of mouse cardiac aorta tissues via Von Kossa staining. Extensive calcium deposition was detected in cardiac aorta tissues from CA1-overexpressing AS and AS mice following MTZ treatment. Little calcification was observed in the aortic tissues of ordinary ApoE [−/−] mice with induced AS.

    Techniques Used: Staining

    Immunofluorescence analysis of macrophages in mouse cardiac aorta tissues. The aortic tissues were stained with DAPI (blue). CD86 (green) represents the M1 macrophage subtype, and CD163 (yellow) represents the M2 macrophage subtype. The expression levels of CD86 were semiquantitatively analyzed. Higher CD86 expression was detected in the aortic tissues of CA1-overexpressing mice than in those of ordinary Apoe [−/−] mice. Increased CD86 expression was detected in the aortic tissues of both CA1-overexpressing mice and ordinary ApoE [−/−] mice when they were induced to AS. ∗ indicates P < 0.05, ∗∗ indicates P < 0.01, ∗∗∗ indicates P < 0.001 and ∗∗∗∗ indicates P < 0.0001.
    Figure Legend Snippet: Immunofluorescence analysis of macrophages in mouse cardiac aorta tissues. The aortic tissues were stained with DAPI (blue). CD86 (green) represents the M1 macrophage subtype, and CD163 (yellow) represents the M2 macrophage subtype. The expression levels of CD86 were semiquantitatively analyzed. Higher CD86 expression was detected in the aortic tissues of CA1-overexpressing mice than in those of ordinary Apoe [−/−] mice. Increased CD86 expression was detected in the aortic tissues of both CA1-overexpressing mice and ordinary ApoE [−/−] mice when they were induced to AS. ∗ indicates P < 0.05, ∗∗ indicates P < 0.01, ∗∗∗ indicates P < 0.001 and ∗∗∗∗ indicates P < 0.0001.

    Techniques Used: Immunofluorescence, Staining, Expressing



    Similar Products

    rabbit anti-mouse ca1 antibody  (Servicebio Inc)
    90
    Servicebio Inc rabbit anti-mouse ca1 antibody
    Establishment of <t>CA1</t> KI ApoE [−/−] C57BL/6 mice via the CRISPR/Cas9 gene re-editing technique. A. Diagram of the establishment of KI mice with CA1 cDNA inserts. An expression cassette with a CAG promoter and CA1-encoding gene was inserted into intron 1 of the Rosa26 locus in the C57BL/6 mouse chromosome via the CRISPR/Cas9 system. B. Representative PCR images showing the successful establishment of CA1-overexpressing KI mice. a. PCR with primer set 1 yielded one band of 296 bp in KI mice. b. PCR with primer set 2 yielded one band of 335 bp in KI mice. c. PCR with primer set 3 yielded one band of 245 bp in the KI mice. KI: knock-in mice; WT: wild type; M: molecular size marker of DNA.
    Rabbit Anti Mouse Ca1 Antibody, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti-mouse ca1 antibody/product/Servicebio Inc
    Average 90 stars, based on 1 article reviews
    rabbit anti-mouse ca1 antibody - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Establishment of CA1 KI ApoE [−/−] C57BL/6 mice via the CRISPR/Cas9 gene re-editing technique. A. Diagram of the establishment of KI mice with CA1 cDNA inserts. An expression cassette with a CAG promoter and CA1-encoding gene was inserted into intron 1 of the Rosa26 locus in the C57BL/6 mouse chromosome via the CRISPR/Cas9 system. B. Representative PCR images showing the successful establishment of CA1-overexpressing KI mice. a. PCR with primer set 1 yielded one band of 296 bp in KI mice. b. PCR with primer set 2 yielded one band of 335 bp in KI mice. c. PCR with primer set 3 yielded one band of 245 bp in the KI mice. KI: knock-in mice; WT: wild type; M: molecular size marker of DNA.

    Journal: Atherosclerosis Plus

    Article Title: ApoE [−/−] CA1-overexpressing knock-in mice aggravated atherosclerosis by increasing M1 macrophages

    doi: 10.1016/j.athplu.2025.03.003

    Figure Lengend Snippet: Establishment of CA1 KI ApoE [−/−] C57BL/6 mice via the CRISPR/Cas9 gene re-editing technique. A. Diagram of the establishment of KI mice with CA1 cDNA inserts. An expression cassette with a CAG promoter and CA1-encoding gene was inserted into intron 1 of the Rosa26 locus in the C57BL/6 mouse chromosome via the CRISPR/Cas9 system. B. Representative PCR images showing the successful establishment of CA1-overexpressing KI mice. a. PCR with primer set 1 yielded one band of 296 bp in KI mice. b. PCR with primer set 2 yielded one band of 335 bp in KI mice. c. PCR with primer set 3 yielded one band of 245 bp in the KI mice. KI: knock-in mice; WT: wild type; M: molecular size marker of DNA.

    Article Snippet: Rabbit anti-mouse CA1 antibody was obtained from Servicebio (China).

    Techniques: CRISPR, Expressing, Knock-In, Marker

    Changes in the body weights of the AS model mice. CA1-overexpressing ApoE [−/−] mice and ordinary ApoE [−/−] mice were induced to AS with high-fat food. These patients were divided into the following groups: normal, AS model, AS model with MTZ treatment and AS model with MTZ preventive-treatment. Each group included 15 mice. Mice with CA1 overexpression generally had greater body weights than did those without CA1 overexpression. The weight of the mice with AS was generally greater than that of the mice without AS, and the weight of the mice with AS was reduced following MTZ treatment and MTZ-preventive treatment.

    Journal: Atherosclerosis Plus

    Article Title: ApoE [−/−] CA1-overexpressing knock-in mice aggravated atherosclerosis by increasing M1 macrophages

    doi: 10.1016/j.athplu.2025.03.003

    Figure Lengend Snippet: Changes in the body weights of the AS model mice. CA1-overexpressing ApoE [−/−] mice and ordinary ApoE [−/−] mice were induced to AS with high-fat food. These patients were divided into the following groups: normal, AS model, AS model with MTZ treatment and AS model with MTZ preventive-treatment. Each group included 15 mice. Mice with CA1 overexpression generally had greater body weights than did those without CA1 overexpression. The weight of the mice with AS was generally greater than that of the mice without AS, and the weight of the mice with AS was reduced following MTZ treatment and MTZ-preventive treatment.

    Article Snippet: Rabbit anti-mouse CA1 antibody was obtained from Servicebio (China).

    Techniques: Over Expression

    Sudan IV staining of mouse cardiac aorta tissues. The number and extent of accumulated lipids in the aorta were semiquantitatively analyzed by calculating lipid accumulation in the whole aorta. The lipid accumulation in the CA1-overexpressing mice was much greater than that in the mice without CA1 overexpression. MTZ treatment significantly decreased the quantity and volume of lipid accumulation. ∗∗ indicates P < 0.01, ∗∗∗ indicates P < 0.001, and ∗∗∗∗ indicates P < 0.0001.

    Journal: Atherosclerosis Plus

    Article Title: ApoE [−/−] CA1-overexpressing knock-in mice aggravated atherosclerosis by increasing M1 macrophages

    doi: 10.1016/j.athplu.2025.03.003

    Figure Lengend Snippet: Sudan IV staining of mouse cardiac aorta tissues. The number and extent of accumulated lipids in the aorta were semiquantitatively analyzed by calculating lipid accumulation in the whole aorta. The lipid accumulation in the CA1-overexpressing mice was much greater than that in the mice without CA1 overexpression. MTZ treatment significantly decreased the quantity and volume of lipid accumulation. ∗∗ indicates P < 0.01, ∗∗∗ indicates P < 0.001, and ∗∗∗∗ indicates P < 0.0001.

    Article Snippet: Rabbit anti-mouse CA1 antibody was obtained from Servicebio (China).

    Techniques: Staining, Over Expression

    HE staining of mouse cardiac aorta tissues. The extent and composition of the aortic lesions were semiquantified by calculating the surface plaque area across the entire aortic area. The number of aortic plaques and wall thickness in CA1-overexpressing mice were greater than those in mice without CA1 overexpression. ∗ indicates P < 0.05, ∗∗ indicates P < 0.01, ∗∗∗ indicates P < 0.001 and ∗∗∗∗ indicates P < 0.0001.

    Journal: Atherosclerosis Plus

    Article Title: ApoE [−/−] CA1-overexpressing knock-in mice aggravated atherosclerosis by increasing M1 macrophages

    doi: 10.1016/j.athplu.2025.03.003

    Figure Lengend Snippet: HE staining of mouse cardiac aorta tissues. The extent and composition of the aortic lesions were semiquantified by calculating the surface plaque area across the entire aortic area. The number of aortic plaques and wall thickness in CA1-overexpressing mice were greater than those in mice without CA1 overexpression. ∗ indicates P < 0.05, ∗∗ indicates P < 0.01, ∗∗∗ indicates P < 0.001 and ∗∗∗∗ indicates P < 0.0001.

    Article Snippet: Rabbit anti-mouse CA1 antibody was obtained from Servicebio (China).

    Techniques: Staining, Over Expression

    Oil Red O staining of mouse cardiac aorta tissues. The number and size of fat deposits were semiquantitatively analyzed. Fat deposition in cardiac aorta tissues was greater in CA1-overexpressing mice with AS than in mice with AS without CA1 overexpression. MTZ treatment significantly decreased the volume and number of fat deposits. ∗ indicates P < 0.05, ∗∗ indicates P < 0.01, ∗∗∗ indicates P < 0.001 and ∗∗∗∗ indicates P < 0.0001.

    Journal: Atherosclerosis Plus

    Article Title: ApoE [−/−] CA1-overexpressing knock-in mice aggravated atherosclerosis by increasing M1 macrophages

    doi: 10.1016/j.athplu.2025.03.003

    Figure Lengend Snippet: Oil Red O staining of mouse cardiac aorta tissues. The number and size of fat deposits were semiquantitatively analyzed. Fat deposition in cardiac aorta tissues was greater in CA1-overexpressing mice with AS than in mice with AS without CA1 overexpression. MTZ treatment significantly decreased the volume and number of fat deposits. ∗ indicates P < 0.05, ∗∗ indicates P < 0.01, ∗∗∗ indicates P < 0.001 and ∗∗∗∗ indicates P < 0.0001.

    Article Snippet: Rabbit anti-mouse CA1 antibody was obtained from Servicebio (China).

    Techniques: Staining, Over Expression

    Biochemical examination of mouse peripheral blood. Compared with those in healthy controls, HDL levels were significantly lower, and AI, LDL, TC and TG levels were elevated in AS mice. The levels of these indices were restored after MTZ treatment. Moreover, the HDL level was significantly lower and the levels of AI, LDL, TC and TG were greater in CA1-overexpressing mice than in ApoE [−/−] mice with normal CA1 expression, regardless of whether these mice were induced to develop AS or treated with MTZ. ∗ indicates P < 0.05, ∗∗ indicates P < 0.01, ∗∗∗ indicates P < 0.001 and ∗∗∗∗ indicates P < 0.0001.

    Journal: Atherosclerosis Plus

    Article Title: ApoE [−/−] CA1-overexpressing knock-in mice aggravated atherosclerosis by increasing M1 macrophages

    doi: 10.1016/j.athplu.2025.03.003

    Figure Lengend Snippet: Biochemical examination of mouse peripheral blood. Compared with those in healthy controls, HDL levels were significantly lower, and AI, LDL, TC and TG levels were elevated in AS mice. The levels of these indices were restored after MTZ treatment. Moreover, the HDL level was significantly lower and the levels of AI, LDL, TC and TG were greater in CA1-overexpressing mice than in ApoE [−/−] mice with normal CA1 expression, regardless of whether these mice were induced to develop AS or treated with MTZ. ∗ indicates P < 0.05, ∗∗ indicates P < 0.01, ∗∗∗ indicates P < 0.001 and ∗∗∗∗ indicates P < 0.0001.

    Article Snippet: Rabbit anti-mouse CA1 antibody was obtained from Servicebio (China).

    Techniques: Expressing

    Immunostaining of CA1 expression in mouse aortic tissues. Immunohistochemistry revealed CA1 expression (brown) in the aortic plaques of the animals with AS (↓). CA1 was also weakly expressed in aortic VSMCs (↓↓). Immunofluorescent immunohistochemistry revealed that CA1 levels were increased in the cardiac aorta tissues of CA1-overexpressing ApoE [−/−] mice, regardless of whether the KI mice had induced AS, compared with those of ordinary ApoE [−/−] mice. The quantified signal is normalized to the total cellularized area. ∗∗∗ indicates P < 0.001 and ∗∗∗∗ indicates P < 0.0001.

    Journal: Atherosclerosis Plus

    Article Title: ApoE [−/−] CA1-overexpressing knock-in mice aggravated atherosclerosis by increasing M1 macrophages

    doi: 10.1016/j.athplu.2025.03.003

    Figure Lengend Snippet: Immunostaining of CA1 expression in mouse aortic tissues. Immunohistochemistry revealed CA1 expression (brown) in the aortic plaques of the animals with AS (↓). CA1 was also weakly expressed in aortic VSMCs (↓↓). Immunofluorescent immunohistochemistry revealed that CA1 levels were increased in the cardiac aorta tissues of CA1-overexpressing ApoE [−/−] mice, regardless of whether the KI mice had induced AS, compared with those of ordinary ApoE [−/−] mice. The quantified signal is normalized to the total cellularized area. ∗∗∗ indicates P < 0.001 and ∗∗∗∗ indicates P < 0.0001.

    Article Snippet: Rabbit anti-mouse CA1 antibody was obtained from Servicebio (China).

    Techniques: Immunostaining, Expressing, Immunohistochemistry

    Calcification of mouse cardiac aorta tissues via Von Kossa staining. Extensive calcium deposition was detected in cardiac aorta tissues from CA1-overexpressing AS and AS mice following MTZ treatment. Little calcification was observed in the aortic tissues of ordinary ApoE [−/−] mice with induced AS.

    Journal: Atherosclerosis Plus

    Article Title: ApoE [−/−] CA1-overexpressing knock-in mice aggravated atherosclerosis by increasing M1 macrophages

    doi: 10.1016/j.athplu.2025.03.003

    Figure Lengend Snippet: Calcification of mouse cardiac aorta tissues via Von Kossa staining. Extensive calcium deposition was detected in cardiac aorta tissues from CA1-overexpressing AS and AS mice following MTZ treatment. Little calcification was observed in the aortic tissues of ordinary ApoE [−/−] mice with induced AS.

    Article Snippet: Rabbit anti-mouse CA1 antibody was obtained from Servicebio (China).

    Techniques: Staining

    Immunofluorescence analysis of macrophages in mouse cardiac aorta tissues. The aortic tissues were stained with DAPI (blue). CD86 (green) represents the M1 macrophage subtype, and CD163 (yellow) represents the M2 macrophage subtype. The expression levels of CD86 were semiquantitatively analyzed. Higher CD86 expression was detected in the aortic tissues of CA1-overexpressing mice than in those of ordinary Apoe [−/−] mice. Increased CD86 expression was detected in the aortic tissues of both CA1-overexpressing mice and ordinary ApoE [−/−] mice when they were induced to AS. ∗ indicates P < 0.05, ∗∗ indicates P < 0.01, ∗∗∗ indicates P < 0.001 and ∗∗∗∗ indicates P < 0.0001.

    Journal: Atherosclerosis Plus

    Article Title: ApoE [−/−] CA1-overexpressing knock-in mice aggravated atherosclerosis by increasing M1 macrophages

    doi: 10.1016/j.athplu.2025.03.003

    Figure Lengend Snippet: Immunofluorescence analysis of macrophages in mouse cardiac aorta tissues. The aortic tissues were stained with DAPI (blue). CD86 (green) represents the M1 macrophage subtype, and CD163 (yellow) represents the M2 macrophage subtype. The expression levels of CD86 were semiquantitatively analyzed. Higher CD86 expression was detected in the aortic tissues of CA1-overexpressing mice than in those of ordinary Apoe [−/−] mice. Increased CD86 expression was detected in the aortic tissues of both CA1-overexpressing mice and ordinary ApoE [−/−] mice when they were induced to AS. ∗ indicates P < 0.05, ∗∗ indicates P < 0.01, ∗∗∗ indicates P < 0.001 and ∗∗∗∗ indicates P < 0.0001.

    Article Snippet: Rabbit anti-mouse CA1 antibody was obtained from Servicebio (China).

    Techniques: Immunofluorescence, Staining, Expressing